Insulin antibody orb385878 from Biorbyt

Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. [RefSeq]

Product Specs
ItemInsulin antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb385878
Quantity20 µg, 100 µg
ApplicationsIHC-P
Conjugate/TagUnconjugated
PurityProtein G Affinity Chromatography
ReactivityHuman, Mouse, Rat
TargetInsulin
IsotypeMouse IgG1, kappa
CloneBCPH1
HostMouse
ImmunogenRecombinant INS protein was used as the immunogen for the Insulin antibody
NCBI Full Gene Nameinsulin
NCBI Gene AliasesIDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the Insulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: Optimal dilution of the Insulin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

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